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Production of 2‐methylisoborneol by Streptomyces violaceusniger and its transformation by selected species of Pseudomonas
Author(s) -
Saadoun Ismail
Publication year - 2005
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410495
Subject(s) - transformation (genetics) , chemistry , zoology , biology , biochemistry , gene
The effect of glucose, glycerol and yeast‐extract (YE) on 2‐methylisoborneol (MIB) production by Streptomyces violaceusniger (C 4 –S strain) was determined. Glycerol as a sole carbon source promoted MIB production was maximum concentration (40.65 µg/l) after 10 days incubation, while glucose favored biomass production. Increasing YE concentration from 0.1 to 1% (W/V) repressed MIB production with 62 and 83% reduction after 10 and 14 days, respectively. Transformation of this odorous compound was investigated using seven different Pseudomonas species. Action of the different Pseudomonas spp. on the C 4 –S extract was followed at 1 hour, 2 hours, 6 hours and 12 hours. Pseudomonas spp. 1, 2, 3 and 5 indicated a positive reaction; however, Pseudomonas spp. 4, 6 and 7 showed no effect. These data suggest that 2‐methylisoborneol can be transformed by Pseudomonas spp. and the test adopted by this study can be applied for screening organisms for their ability to transform MIB. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)