Premium
Identification of Brucella species isolated from proven brucellosis patients in Izmir, Turkey
Author(s) -
Köse Şükran,
Kiliç Selçuk,
Özbel Yusuf
Publication year - 2005
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410469
Subject(s) - brucellosis , brucella , brucella abortus , identification (biology) , biology , microbiology and biotechnology , virology , botany
In many parts of the world, brucellosis has significantly decreased, but it is still a problem in some regions of Turkey. Totally, 11 Brucella strains isolated from the blood cultures of patients with presumptive diagnosis of brucellosis were identified to species and biovar level. For species identification, (i) CO 2 requirements of isolates; (ii) detection of urease activation; (iii) detection of H 2 S production; (iv) dye sensitivities (thionine and basic fuchsin); (v) susceptibility to Brucella phage and (vi) agglutination with monospecific antisera (A and M) were performed. Ten out of 11 isolates were identified as B. melitensis biovar 3 and one out of 11 were identified as B. melitensis biovar 1. The sensitivity of isolates to antibiotics was determined by the E‐test. All isolates were found to be sensitive to doxycycline, rifampin, ciprofloxacin and cephtriaxone. Only one out of 11 isolates was found to be semi‐sensitive to trimethoprim/sulfamethoxazole (cotrimoxazole). (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)