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Role of different additives and metallic micro minerals on the enhanced citric acid production by Aspergillus niger MNNG‐115 using different carbohydrate materials
Author(s) -
Ali Sikander,
Haq Ikramul
Publication year - 2005
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410460
Subject(s) - citric acid , aspergillus niger , chemistry , zinc , bagasse , starch , sugar , nuclear chemistry , cobalt , food science , biochemistry , inorganic chemistry , organic chemistry , microbiology and biotechnology , biology
The present investigation deals with the promotry effect of different additives and metallic micro minerals on citric acid production by Aspergillus niger MNNG‐115 using different carbohydrate materials. For this, sugar cane bagasse was fortified with sucrose salt medium. Ethanol and coconut oil at 3.0% (v/w) level increased citric acid productivity. Flouroacetate at a concentration of 1.0 mg/ml bagasse enhanced the yield of citric acid significantly. However, the addition of ethanol and flouroacetate after 6 h of growth gave the maximum conversion of available sugar to citric acid. In another study, influence of some metallic micro‐minerals viz. copper sulphate, molybdenum sulphate, zinc sulphate and cobalt sulphate on microbial synthesis of citric acid using molasses medium was also carried out. It was found that copper sulphate and molybdenum sulphate remarkably enhanced the production of citric acid while zinc sulphate was not so effective. However, cobalt sulphate was the least effective for microbial biosynthesis of citric acid under the same experimental conditions. In case of CuSO 4 , the strain of Aspergillus niger MNNG‐115 showed enhanced citric productivity with experimental (9.80%) over the control (7.54%). In addition, the specific productivity of the culture at 30 ppm CuSO 4 ( Q p = 0.012a g/g cells/h) was several folds higher than other all other concentrations. All kinetic parameters including yield coefficients and volumetric rates revealed the hyper productivity of citric acid by CuSO 4 using blackstrap molasses as the basal carbon source. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)