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Screening of bacterial antagonists for biological control of Phytophthora blight of pepper
Author(s) -
Rajkumar M.,
Lee Wang Hyu,
Lee Kui Jae
Publication year - 2005
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410445
Subject(s) - phytophthora capsici , pepper , seedling , biology , blight , antagonism , rhizosphere , horticulture , pseudomonas , agar , rhizobacteria , colletotrichum capsici , rhizoctonia solani , shoot , botany , bacteria , fungicide , biochemistry , genetics , receptor
The aim of this study was to assess the potential of bacterial antagonists to control Phytophthora blight of pepper caused by P. capsici using different screening methods. Among a collection of fluorescent pseudomonas isolated from the rhizosphere of pepper, twelve isolates were initially selected based on dual culture assay on potato dextrose agar and corn meal agar. Further, these twelve isolates were screened for the reduction of disease severity caused by P. capsici using detached leaves and seedling assay. Most of the antagonists showed varying levels of antagonism against P. capsici in both detached leaves and seedlings assay. In addition, few isolates increased shoot and root length of pepper in seedling assays. Among them, isolate PS119 showing highest ability to reduce the disease severity in the in vitro seedling assay was found to be the most efficient antagonists against P. capsici in the in vivo biological control tests. These results indicate that the in vitro seedling assay can be used as a rapid and more accurate technique for the selection of promising biocontrol agents against P. capsici . (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)