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Phylogenetic study on Shiraia bambusicola by rDNA sequence analyses
Author(s) -
Cheng TianFan,
Jia XiaoMing,
Ma XiaoHang,
Lin Haiping,
Zhao YuHua
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410434
Subject(s) - phylogenetic tree , biology , maximum parsimony , evolutionary biology , primer (cosmetics) , phylogenetics , ribosomal dna , botany , genetics , clade , gene , chemistry , organic chemistry
In this study, 18S rDNA and ITS‐5.8S rDNA regions of four Shiraia bambusicola isolates collected from different species of bamboos were amplified by PCR with universal primer pairs NS1/NS8 and ITS5/ITS4, respectively, and sequenced. Phylogenetic analyses were conducted on three selected datasets of rDNA sequences. Maximum parsimony, distance and maximum likelihood criteria were used to infer trees. Morphological characteristics were also observed. The positioning of Shiraia in the order Pleosporales was well supported by bootstrap, which agreed with the placement by Amano (1980) according to their morphology. We did not find significant inter‐hostal differences among these four isolates from different species of bamboos. From the results of analyses and comparison of their rDNA sequences, we conclude that Shiraia should be classified into Pleosporales as Amano (1980) proposed and suggest that it might be positioned in the family Phaeosphaeriaceae . (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)