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Tat dependent export of E. coli phytase AppA by using the PhoD‐specific transport system of Bacillus subtilis
Author(s) -
Gerlach Roman,
Pop Ovidiu,
Müller Jörg P.
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410423
Subject(s) - bacillus subtilis , signal peptide , escherichia coli , heterologous , biochemistry , extracellular , proteases , fusion protein , chemistry , biology , enzyme , microbiology and biotechnology , recombinant dna , bacteria , gene , genetics
It has been shown recently that the twin‐arginine signal peptide of Bacillus subtilis phosphodiesterase PhoD (SP PhoD ) can mediate Tat dependent transport of proteins via its specific Tat‐transport components. In order to test the use of Tat dependent transport signals for heterologous product synthesis, Escherichia coli phytase AppA was expressed under control of PhoD‐specific export signals in B. subtilis . Induction of Tat components TatA d /TatC d was mediated by using a functionally altered PhoR/PhoP signal transduction system which regulates the expression of these components. AppA was highly susceptible to host specific extracellular proteases. Expression of appA in B. subtilis wprA strain resulted in the stable production of AppA. A fusion protein consisting of SP PhoD and mature AppA remained unprocessed, while introduction of the AppA signal peptidase cleavage site resulted in efficient processing of the fusion protein. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)