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Isolation of Citrobacter sp. mutants defective in decolorizing malachite green
Author(s) -
Jang MoonSun,
Lee YoungMi,
Choi YongLark,
Cho YoungSu,
Kim CheorlHo,
Lee YoungChoon
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200410384
Subject(s) - malachite green , mutant , biology , gene , transposable element , insertion sequence , microbiology and biotechnology , biochemistry , genetics , chemistry , organic chemistry , adsorption
To identify genes involved in the decolorization of malachite green, random mutants generated by transposon insertion in the malachite green‐decolorizing bacterium, Citrobacter sp. were isolated. The resulting mutant bank yielded 24 mutants with complete defects in their abilities to decolorize malachite green. Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in 7 mutants, which appeared to have insertions at different sites of the chromosome. The Tn5‐inserted genes were isolated and the DNA sequence flanking Tn5 was determined. Based on a sequence database, the putative protein products encoded by the mg genes were identified as follows. mg3 , an ABC transporter homolog; mg6 , a LysR‐type regulatory protein; m11 , an oxidoreductase; mg17 , a MalG protein in the maltose transport system; and mg21 , a sugar kinase. The deduced sequences from two mg genes ( mg 7 and mg18 ) showed no significant similarity to any protein with a known function, suggesting that these two mg genes encode unidentified proteins that are responsible for the decolorization of malachite green. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)