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Xylanolytic complex from Aspergillus giganteus : production and characterization
Author(s) -
Coelho Glauciane Danusa,
Carmona Eleonora Cano
Publication year - 2003
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200390030
Subject(s) - aspergillus , characterization (materials science) , biology , microbiology and biotechnology , nanotechnology , materials science
An Aspergillus giganteus strain was isolated as an excellent producer of xylanase associated with low levels of cellulase. Optimal xylanase production was obtained in liquid Vogel medium containing xylan as carbon source, pH 6.5 to 7.0, at 25 °C and under shaking at 120 rpm during 84h. Among the several carbon sources tested, higher xylanase production was verified in xylan, xylose, sugar‐cane bagasse, wheat bran and corn cob cultures, respectively. Optimal conditions for activity determination were 50 °C and pH 6.0. The xylanolytic complex of A. giganteus showed low thermal stability with T 50 of 2 h, 13 min and 1 min when it was incubated at 40, 50 and 60 °C, respectively, and high stability from pH 4.5 to 10.5, with the best interval between 7.0 to 7.5. This broad range of stability in alkali pH indicates a potential applicability in some industrial processes, which require such condition. Xylanolytic activity of A. giganteus was totally inhibited by Hg +2 , Cu +2 and SDS at 10 m M . The analysis of the products from the oat spelts xylan hydrolysis through thin‐layer chromatography indicated endoxylanase activity, lack of debranching enzymes and β‐xylosidase activity in assay conditions.