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Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe
Author(s) -
Gazdag Zoltán,
Pócsi István,
Belágyi József,
Emri Tamás,
Blaskó Ágnes,
Takács Krisztina,
Pesti Miklós
Publication year - 2003
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200390018
Subject(s) - schizosaccharomyces pombe , menadione , chemistry , glutathione , chromate conversion coating , mutant , oxidative stress , glutathione reductase , schizosaccharomyces , biochemistry , reductase , enzyme , gene , organic chemistry , chromium , glutathione peroxidase
The stable Cr(VI)‐tolerant chr1‐66T mutant of Schizosaccharomyces pombe , which carries one simple gene mutation responsible for Cr(VI) tolerance, accumulated and reduced the chromate anion (CrO 4 2– ) significantly more slowly than did its parental strain 6chr + . The mutant chr1‐66T proved to be sensitive to oxidative stressors such as H 2 O 2 , menadione, tert ‐butyl hydroperoxide and Cd 2+ . Both the Cr(VI) tolerance and the oxidative stress sensitivity were attributed to a decreased specific glutathione reductase activity. These effects were also enhanced with a decrease in the specific mitochondrial Mn‐SOD activity.