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Identification of a major protein upon phosphate starvation of Pseudomonas aeruginosa PAO1
Author(s) -
Madhusudhan Kunapuli T.,
McLaughlin Robert,
Komori Naoka,
Matsumoto Hiroyuki
Publication year - 2003
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200390002
Subject(s) - phosphate , bacteria , biochemistry , pseudomonas aeruginosa , biology , pseudomonas , edman degradation , amino acid , operon , chemistry , chromatography , microbiology and biotechnology , escherichia coli , peptide sequence , gene , genetics
To understand the physiology of non‐differentiating bacteria exposed to nutrient deprivation and stress, various approaches have been employed in combination with detailed analysis of protein synthesis pattern. In this study, separation of proteins from clarified cell extracts of Pseudomonas aeruginosa PAO1 grown under phosphorus limiting conditions was achieved by high resolution two‐dimensional gel electrophoresis (2‐DE). Limitation of phosphate in the growth medium revealed significant differences in the 2‐DE pattern of proteins between phosphate starved cells and an unstarved control. A major protein identified as PstS, a phosphate binding protein of the pts operon was exclusively found on 2‐DE gels of phosphate starved bacteria. The identity of protein was established based on the results of Edman degradation, amino acid analysis and mass spectrometry. PstS was also found in other pseudomonads, and therefore, it can be used as a landmark protein in proteomic studies. Additionally, we propose utilizing pstS of pseudomonads for testing bioavailable phosphate from soils and water streams.