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Control of pyrimidine biosynthesis in “ Pseudomonas alkanolytica ” ATCC 21034
Author(s) -
West Thomas P.
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310373
Subject(s) - biosynthesis , pyrimidine metabolism , pseudomonas , pyrimidine , chemistry , biochemistry , microbiology and biotechnology , bacteria , biology , enzyme , genetics , purine
The control of de novo pyrimidine biosynthesis in the industrially important patent strain “ Pseudomonas alkanolytica ” ATCC 21034 was investigated. Uracil supplementation of succinate‐grown “ P . alkanolytica ” cells produced the greatest depression of the de novo pyrimidine biosynthetic pathway enzyme activities. After the pyrimidine limitation of a “ P . alkanolytica ” orotate phosphoribosyltransferase mutant strain grown on succinate, the pyrimidine biosynthetic pathway enzyme activities were derepressed. The pyrimidine biosynthetic pathway enzyme aspartate transcarbamoylase in “ P . alkanolytica ” was inhibited by pyrophosphate, cytidine 5′‐triphosphate (CTP), uridine 5′‐triphosphate (UTP), and guanosine 5′‐triphosphate (GTP). (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)