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Production of laccase isoforms by Pleurotus pulmonarius in response to presence of phenolic and aromatic compounds
Author(s) -
Giatti Marques De Souza Cristina,
Kirst Tychanowicz Giovana,
Farani De Souza Daniela,
Peralta Rosane Marina
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310365
Subject(s) - laccase , vanillin , ferulic acid , chemistry , inducer , lignin , phenols , guaiacol , organic chemistry , food science , fungus , biochemistry , enzyme , botany , biology , gene
The effect of several phenolic and aromatic monomers structurally‐related to lignin on production of laccase by the white rot fungus P. pulmonarius ( Fr .) Quélet has been studied. In the absence of an inducer, laccase was maximally produced after depletion of carbon and nitrogen sources. Among 15 phenolic and aromatic compounds tested, ferulic acid and vanillin were the most efficient inducers, increasing the production of laccase activity up to 10 times. A mixture of ferulic acid and vanillin was more efficient to induce the production of laccase than the isolated phenolics. At least three laccase isoforms designated as lcc 1, lcc 2 and lcc 3 were identified by eletrophoretic analysis of P. pulmonarius culture filtrates. The lcc 1 and lcc 2 isoforms were produced by non‐induced cultures, while lcc 3 was found only in induced‐culture filtrates. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)