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Nucleotide sequence analysis of the recA gene and discrimination of the three isolates of urease‐positive thermophilic Campylobacter (UPTC) isolated from seagulls ( Larus spp.) in Northern Ireland
Author(s) -
Matsuda M.,
Tai K.,
Moore J. E.,
Millar B. C.,
Murayama O.
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310346
Subject(s) - amplicon , biology , gene , genetics , nucleic acid sequence , nucleotide , microbiology and biotechnology , polymerase chain reaction
Nucleotide sequencing after TA cloning of the amplicon of the almost‐full length recA gene from three strains of UPTC (A1, A2, and A3) isolated from seagulls in Northern Ireland, the phenotypical and genotypical characteristics of which have been demonstrated to be indistinguishable, clarified nucleotide differences at three nucleotide positions among the three strains. In conclusion, the nucleotide sequences of the recA gene were found to discriminate among the three strains of UPTC, A1, A2, and A3, which are indistinguishable phenotypically and genotypically. Thus, the present study strongly suggests that nucleotide sequence data of the amplicon of a suitable gene or region could aid in discriminating among isolates of the UPTC group, which are indis‐ tinguishable phenotypically and genotypically. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)