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Isolation and characterization of cerebrosides of the hydrocarbon‐assimilating yeast Yarrowia lipolytica
Author(s) -
Rupčić Jasminka,
Georgiù Kata,
Marić Vladimir
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310337
Subject(s) - yarrowia , cerebroside , yeast , chemistry , glycolipid , fatty acid , biochemistry , composition (language) , chromatography , hexadecane , column chromatography , thin layer chromatography , sphingolipid , organic chemistry , philosophy , linguistics
Yarrowia lipolytica yeast was grown batchwise on n ‐hexadecane as the carbon and energy source. Two cerebroside species were quantitatively isolated from sphingolipid fractions of total lipids by a combination of column chromatography and preparative high‐performance thin‐layer chromatography. The cerebroside content accounted for 1.3% of the total cell lipids. Glucose was detected as the sole sugar constituent in cerebrosides. The fatty acid composition of cerebrosides was characterized by a high proportion of hydroxylated long‐chain saturated fatty acids. The major fatty acids were h16:0 and 16:0. The long‐chain bases composition shows a preponderance of trihydroxy bases and a small amount of dihydroxy bases. The striking finding was a high proportion of 19‐phytosphingosine. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)