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Inhibition of patulin production by Penicillium expansum cultured with neem ( Azadirachta indica ) leaf extracts
Author(s) -
Aparecida Galerani Mossini Simone,
De Oliveira Kesia Pires,
Kemmelmeier Carlos
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310332
Subject(s) - patulin , azadirachta , penicillium expansum , meliaceae , mycotoxin , penicillium , fungicide , biology , penicillium chrysogenum , botany , chemistry , horticulture , postharvest
Aqueous extract of the leaves of neem [ Azadirachta indica A. Juss (Meliaceae)] was tested in vitro for antifungal activity against Penicillium expansum . Patulin production was inhibited during cultivation, when concentrations higher than 50 mg/ml of neem leaf extract was added to the culture medium. Analyses of mycotoxin production were performed by TLC Abbreviations used: TLC, Thin layer chromatography; HPLC, High‐performance liquid chromatography; UEM, Universidade Estadual de Maringá [State University of Maringá]; IAPAR, Instituto Agrônomico do Paraná [Agronomy Institute of Paraná]. and HPLC. Fungal growth and colony characteristics, in the presence of the extract, were investigated and compared with extract‐free cultures. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)