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Biosynthesis of tannase and gallic acid from tannin rich substrates by Rhizopus oryzae and Aspergillus foetidus
Author(s) -
Mukherjee Gargi,
Banerjee Rintu
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310317
Subject(s) - tannase , gallic acid , rhizopus oryzae , terminalia chebula , tannin , aspergillus oryzae , fermentation , food science , rhizopus , solid state fermentation , chemistry , biochemistry , traditional medicine , medicine , antioxidant
Modified solid‐state fermentation (MSSF) of tannin‐rich substrates for production of tannase and gallic acid was carried out using two fungal cultures, Rhizopus oryzae (RO IIT RB‐13, NRRL 21498) and Aspergillus foetidus (GMRB013 MTCC 3557). The tannin rich substrates included powdered fruits of Terminalia chebula and Caesalpinia digyna pod cover powder. The different environmental parameters for the maximum production of tannase and gallic acid were optimized through media engineering. The highest yield of tannase and gallic acid was obtained after 60 h in case of Rhizopus oryzae and after 72 h by Aspergillus foetidus with 3 ml of induced inoculum. The optimum initial pH of the fermentation was found to be 4.5 in case of Rhizopus oryzae and 5.0 for Aspergillus foetidus . MSSF was carried out at the optimum conditions of 30 °C and 80% relative humidity. Collectively, the data reveal the potential of the modified solid‐state fermentation process for the production of tannase and gallic acid from tannin‐rich substrates with R. oryzae and A. foetidus .