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Examination of membrane protein expression in Paracoccus denitrificans by two‐dimensional gel electrophoresis
Author(s) -
Bouchal Pavel,
Kučera Igor
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310312
Subject(s) - paracoccus denitrificans , periplasmic space , denitrifying bacteria , bacterial outer membrane , bacteria , membrane protein , membrane , two dimensional gel electrophoresis , gel electrophoresis , biochemistry , chemistry , electrophoresis , biology , chromatography , proteomics , microbiology and biotechnology , enzyme , escherichia coli , denitrification , gene , genetics , organic chemistry , nitrogen
The well‐known metabolic versatility of the soil bacterium Paracoccus denitrificans poses a challenge for modern proteomic approaches. We describe here improved preparation conditions that allow good separation and quantitative analyses of hundreds of membrane or periplasmic proteins. To illustrate this optimized procedure, the results of a screening for membrane proteins associated predominantly with aerobic or anaerobic (denitrifying) modes of growth are presented.