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Extracellular chitinase production by Trichoderma harzianum in submerged fermentation
Author(s) -
Sandhya C.,
Adapa Leela Krishna,
Nampoothiri K. Madhavan,
Binod P.,
Szakacs George,
Pandey Ashok
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310284
Subject(s) - chitinase , trichoderma harzianum , chitin , fermentation , mycelium , food science , fungus , carbon source , chemistry , botany , biology , biochemistry , enzyme , biological pest control , chitosan
Extra‐cellular chitinase production by a chitinolytic fungus Trichoderma harzianum TUBF 966 using submerged fermentation was studied. Colloidal chitin (1.5% w/v) was used as sole carbon source. Maximum chitinase production (14.7 U/ml) was obtained when fermentation was carried out at 30 °C for 96 h using 72 h old mycelium in a medium containing colloidal chitin 1.5% (w/v) as carbon source and 0.42 (% w/v) peptone as nitrogen source (pH 5.5). Supplementation of additional carbon sources (0.75% w/v) showed no further enhancement in chitinase production while supplementation of nitrogen sources (0.42% w/v) such as peptone and tryptone in the fermentation medium showed a marked increase in production. The process parameters that controlled chitinase production by the fungus were studied and presented here.