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Evidences of high carbon catabolic enzyme activities during sporulation of Pleurotus ostreatus (Florida)
Author(s) -
Chakraborty Tapas K.,
Das Nirmalendu,
Mukherjee Mina
Publication year - 2003
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310275
Subject(s) - catabolism , pentose phosphate pathway , biochemistry , enzyme , dehydrogenase , trehalose , carbohydrate metabolism , anabolism , biology , pleurotus ostreatus , metabolism , phosphogluconate dehydrogenase , citric acid cycle , glycolysis , pentose , catabolite repression , metabolic pathway , xylose , glucose 6 phosphate dehydrogenase , food science , gene , fermentation , mushroom , mutant
Measurements of the specific activities of the representative enzymes of different pathways linked to carbohydrate metabolism indicate that glycolysis and TCA cycles are the major route of carbohydrate catabolism in the sporulating phase of fruiting body development in Pleurotus ostreatus . Enzymes of the pentose phosphate pathway always showed lower specific activities as compared to those of the enzymes of the glycolytic pathway. The activity of NADP linked glutamate dehydrogenase which is known to be an anabolic enzyme decreased drastically in sporulating fruiting bodies and in spore containing gill tissue (spore bearing structure). Mannitol dehydrogenase activity declined significantly in the sporulating phase of P. ostreatus . The high rate of metabolism during sporulation was further supported by a lower rate of gluconeogenesis at this stage. Concentrations of all the major sugars of the fruiting body (mannitol, glucose and trehalose) decreased in the mature fruiting body and gill tissue. This indicated high catabolic activities at this stage of development.