Premium
Electrophoretic karyotype of two Micromucor species
Author(s) -
Nagy Ágnes,
Pesti Miklós,
Galgóczy László,
Vágvölgyi Csaba
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310272
Subject(s) - karyotype , schizosaccharomyces pombe , biology , chromosome , protoplast , genome , dna , genome size , genetics , electrophoresis , nuclear dna , yeast , saccharomyces cerevisiae , microbiology and biotechnology , gene , mitochondrial dna
Electrophoretic karyotype analysis was applied to obtain information on the organisation and intrageneric variability of the nuclear genome in three Micromucor isolates of two different species (M. isabellina and M. ramanniana) . A protoplast formation protocol, conditions for the preparation of highly‐intact chromosome‐size DNA molecules and for the separation of DNA molecules were established. The chromosomal banding patterns revealed substantial variability among the isolates: 11 to 14 chromosomal mobility groups were resolved. The DNA in the Micromucor chromosomes were rather small; their estimated sizes were calculated to be between 2.60 and 0.4 Mb. Using Saccharomyces cerevisiae and Schizosaccharomyces pombe as size standard, the minimum total genome sizes were estimated to be between 24.19 and 24.9 Mb.