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Chloride transport in the vegetative mycelia of filamentous fungus Trichoderma viride
Author(s) -
Šimkovič M.,
Pokorný R.,
Hudecová D.,
Varečka Ľ.
Publication year - 2004
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310261
Subject(s) - valinomycin , vanadate , chemistry , antiporter , ionophore , trichoderma viride , mycelium , oligomycin , chloride , nuclear chemistry , sodium , atpase , biophysics , membrane potential , membrane , biochemistry , botany , enzyme , food science , biology , organic chemistry
The influx of chlorides into Trichoderma viride vegetative submerged mycelium was measured by means of the radionuclide 36 Cl − . It was found that the 36 Cl − influx was time‐dependent (the steady‐state was established with t 1/2 = 25 min at 25 °C), pH‐dependent (with pH optimum between 4–5.5), temperature‐dependent (at about 15 °C), and concentration‐dependent (K M (Cl – )= 47.6 ± 4.2 μmol · l −1 ; J max = 11.5 ± 0.7 pmol(Cl − ) · min −1 · mg dry mass −1 ). The 36 Cl − influx was inhibited by Br − but not F − , I − , SO 4 2− , HPO 3 2− and HCO 3 − . The presence of vanadate (P‐type ATPase inhibitor) moderately stimulated the 36 Cl − influx but the presence valinomycin (electrogenic K + ionophore), salicylate (known to release Ca 2+ from Trichoderma viride internal stores) were without effect on the 36 Cl − influx. The results suggest that the 36 Cl − influx is mediated by a carrier and that the transport is electroneutral, probably Cl − /OH − antiport. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)