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Characterization by restriction fragment length polymorphism and plasmid profiling of Vibrio tapetis strains
Author(s) -
Le Chevalier Patrick,
Le Boulay Christine,
Paillard Christine
Publication year - 2003
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.200310241
Subject(s) - restriction fragment length polymorphism , biology , ecori , plasmid , restriction enzyme , hindiii , genetics , vibrio , vibrionaceae , microbiology and biotechnology , phenotype , restriction map , terminal restriction fragment length polymorphism , restriction fragment , southern blot , genotype , bacteria , dna , gene
A total of nine Vibrio tapetis strains acquired from France, the United Kingdom and Norway were studied for their plasmid content. All the isolates contained from two to four large plasmids, ranging from approximately 60 to 100 kpb. in size. These plasmids were subsequently subjected to restriction fragment length polymorphism analysis (RFLP). Using the Eco RI enzyme, three different restriction patterns were demonstrated, two of which were closely related. On the basis of RFLP patterns, the strain from Norway differed noticeably from the French and British strains.