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Formation of additional contacts of chromosome with membrane in the process of DNA repair synthesis in bacterial cells
Author(s) -
Bezlepkin V. G.,
Malinovsky Yu. Yu.,
Gaziev A. I.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830231002
Subject(s) - dna polymerase , dna , microbiology and biotechnology , dna clamp , dna polymerase ii , dna gyrase , dna synthesis , dna repair , dna replication , polymerase , circular bacterial chromosome , biology , chemistry , dna polymerase delta , biochemistry , polymerase chain reaction , gene , escherichia coli , reverse transcriptase
An increase in the amount of membrane‐bound DNA was found in B. subtilis cells with UV‐induced DNA repair synthesis as compared to untreated cells. It was shown that DNA repair synthesis occurred in DNA membrane complexes (DMC) formed during UV‐irradiation. UV‐induced formation of DMC was observed in cells of wild type strains which were capable of repairing damaged DNA but not in a mutant defective in DNA‐polymerase I. It was demonstrated that DNA‐polymerase I is located on the membrane of B. subtilis cells. This suggested a participation of DNA‐polymerase I in binding of the chromosome to the membrane in UV‐irradiated cells. UV‐induced DMC did not dissociate when the cells were treated with inhibitors of DNA‐gyrase. It, therefore, was qualitatively different from the DMC found during replication. The mechanisms of binding of the damaged DNA to the membrane in UV‐irradiated cells of B. subtilis are discussed.