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Bildung und Isolierung des Phytotoxins Tentoxin aus Alternaria alternata
Author(s) -
Liebermann B.,
Oertel B.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830230810
Subject(s) - alternaria alternata , mycelium , phytotoxin , absorbance , bioassay , fermentation , chemistry , chlorosis , mycotoxin , chromatography , toxin , botany , biology , food science , biochemistry , genetics
A chlorosis‐inducing phytoeffector from culture filtrate of Alternaria alternata (FRIES) KEISSLER, whose mycelium was grown in synthetic media, was isolated with good yields (50 mg/l) and identified as tentoxin. In addition to this compound, purification on Sephadex G‐15 yielded several byproducts with absorbance at 270 nm. This procedure of tentoxin isolation is more effective than traditional ones. The phytotoxin, whose production depends on fermentation‐temperature, can be determined quantitatively in a good agreement by bioassay or spectrophotometry.