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Effects of cycloheximide on the temperature profile of Sacharomyces cerevisiae
Author(s) -
MadeiraLopes A.,
van Uden N.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830230717
Subject(s) - cycloheximide , arrhenius plot , arrhenius equation , saccharomyces cerevisiae , yeast , biophysics , chemistry , biology , biochemistry , protein biosynthesis , activation energy
T max , the maximum temperature for growth of Saccharomyces cerevisiae , decreased linearly with increasing concentrations of cycloheximide added to the medium, to about 20 °C at 2.5 μg ml −1 . In this concentration range thermal death was not enhanced. The ARRHENIUS plot of growth was shifted to lower temperatures as a function of the cycloheximide concentrations and became dissociated from the ARRHENIUS plot of thermal death. It was concluded that the target site of cycloheximide, the cytoplasmic ribosome, is not identical with the physiological T max site of S. cerevisiae and that the binding of cycloheximide to its target sites is strongly enhanced by the temperature.