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Reinigung und Gewinnung des arom ‐Aggregates von Schizosaccharomyces pombe
Author(s) -
Bode R.,
Kunze G.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830230503
Subject(s) - schizosaccharomyces pombe , neurospora crassa , chemistry , size exclusion chromatography , euglena gracilis , electrophoresis , chromatography , biochemistry , biology , enzyme , yeast , saccharomyces cerevisiae , chloroplast , gene , mutant
The five enzymes that catalyzing steps two through six in the prechorismate polyaromatic amino acid biosynthetic pathway are physically associated and have been purified up to 400‐fold from Schizosaccharomyces pombe. The native arom aggregate has a molecular weight of approx. 140,000–145,000 based on gel filtration, glycerol‐density‐gradient centrifugation, and polyacrylamide‐gel electrophoresis in the presence of sodium dodecyl sulphate. Similarities between the S. pombe arom aggregate and that of Neurospora crassa and Euglena gracilis are discussed.