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Elektronenmikroskopische Untersuchung an Komplexen von DNA mit der Restriktionsendonuclease Sal GI
Author(s) -
Johannssen W.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830230308
Subject(s) - restriction enzyme , microbiology and biotechnology , chemistry , dna , recognition sequence , endonuclease , plasmid , biology , biochemistry
The binding properties of the type II restriction endonuclease Sal GI to the plasmid DNA pGW 10 has been investigated by electron microscopic studies. Samples were spread by the BCA technique. In the presence of magnesium, Sal GI binds as dimers and tetramers to the specific recognition site 5′‐G‐T‐C‐G‐A‐C‐3′ and with lower rate to the sequence 5′‐G‐T‐C‐A‐A‐C‐3′, which represents the recognition site of the restriction endonucleases Hind II and Hinc II.