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Lytic enzyme activity in autolysing mycelium of Aspergillus niger
Author(s) -
Lahoz R.,
Reyes Fuensanta,
Gómez P.,
Martinez M. J.
Publication year - 1983
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19830230104
Subject(s) - autolysis (biology) , aspergillus niger , mycelium , acid phosphatase , enzyme , esterase , enzyme assay , biochemistry , invertase , chemistry , cellulase , alkaline phosphatase , amylase , biology , food science , botany
We have studied changes in the activity of some lytic enzymes contained in mycelium of Aspergillus niger in cultures relative to the autolytic phase of growth. Acid phosphatase, polygalacturonidase and α‐amylase activity reached its highest level (40.7, and 8 U/sample, respectively) at the initiation of the autolytic phase of growth. 1,3‐ß‐Glucanase and ß‐N‐acetylglucosaminidase reached its highest level (3.5 and 2 U/sample, respectively) during the first days of autolysis. Alkaline phosphatase, cellulase, invertase, esterase, chitinase and proteolytic activity is also present in autolysing mycelium of A. niger , though comparatively low. Their maximum activity coincided with the beginning of the autolytic phase of growth. In all enzymes studied here, as autolysis proceeded, enzyme activity decreased by about 90%. Only esterase activity remained nearly constant throughout the whole period of autolysis described here.