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Genetic segregation in a high‐yielding streptomycin‐producing strain of Streptomyces griseus
Author(s) -
Roth M.,
Schwalenberg B.,
Reiche R.,
Noack D.,
Geuther R.,
Eritt I.
Publication year - 1982
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19820220807
Subject(s) - streptomyces griseus , streptomycin , mycelium , strain (injury) , protoplast , biology , spore , glycerol , reversion , sporangium , streptomyces , microbiology and biotechnology , botany , biochemistry , phenotype , bacteria , genetics , gene , antibiotics , anatomy
The streptomycin‐producing Streptomyces griseus HP spontaneously segregated non‐reverting derivatives with altered phenotypes. Clones characterized by increased spore formation and decreased streptomycin production were found. Two other types of derivatives were defective in aerial mycelium and streptomycin formation as well, but differed in the capacity to synthesize a yellow pigment. These derivatives were examined with respect to further properties. The stability of S. griseus HP was investigated in relation to conditions of continuous culture. Both at 26 and 30°C, under glycerol and NH 4 Cl limitation a rapid segregation and enrichment of streptomycin‐non‐producing derivatives occurred. At 34°C and glycerol limitation segregation began only after about 35 generations of continuous culture. In NH 4 Cl‐limited chemostats the original strain was stable during 80 generations. In the course of the continuous culture experiments it was shown that the onset of genetic segregation with mycelia can be detected before it becomes obvious in colonies grown from the mycelia. This was achieved by fractionation of the mycelia by protoplast formation and subsequent plating on regeneration medium allowing colony growth and differentation.