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Transformation of 12‐deoxycardenolids with Streptomyces purpurascens
Author(s) -
Albrecht K.,
Máté G.,
Láng T.
Publication year - 1982
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19820220702
Subject(s) - digitoxin , digoxin , fermentation , streptomyces , transformation (genetics) , solvent , chemistry , substrate (aquarium) , biochemistry , biology , medicine , bacteria , genetics , heart failure , ecology , gene
Streptomyces purpurascens transforms primary and secondary 12‐deoxycardenolids into digoxin, 7β‐hydroxy‐digitoxin and 7β‐hydroxy‐digoxin, respectively. A metabolic pathway is proposed for cardenolid transformations and the enzymes participating in it are investigated. The substrate concentration in the medium can be increased by using a water‐miscible solvent of electron donor character. The newly developed procedure yields 0.5 g/l −1 of digoxin in a five‐day fermentation.