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Factors regulating the steroid 11‐hydroxylation by non‐germinating spores of Cunninghamella elegans (LENDNER)
Author(s) -
Jaworski A.,
Sedlaczek L.,
Wilmańska D.,
Sasiak A.,
Strycharska A.
Publication year - 1982
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19820220506
Subject(s) - spore , hydroxylation , germination , biochemistry , biology , glyoxylate cycle , microbiology and biotechnology , enzyme , botany
In the presence of malate or citrate sporangiospores of C. elegans were able to hydroxylate cortexolone with a rate twofold exceeding that of the control, water suspended spores. Analysis of the intracellular nicotinamide coenzyme pools revealed an increased NADPH: (NADP + + NADPH) ratio, indicating more effective NADPH‐generating systems in malate‐ or citrate‐stimulating spores. Swollen spores remaining in the pregermination state, retained higher cortexolone‐hydroxylating activity in the absence of malate and citrate. In these spores degradation of endogenous alanine and glutamic acid was observed. Possible NADPH‐generating systems in C. elegans sporangiospores were discussed.