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Mikrobielle Seitenkettenabspaltung von Phenylharnstoffen und Carbonsäureaniliden
Author(s) -
Lechner U.,
Straube G.,
Köhler M.
Publication year - 1982
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19820220404
Subject(s) - chemistry , hydrolysis , aniline , amidase , amide , substrate (aquarium) , peptide bond , medicinal chemistry , stereochemistry , organic chemistry , enzyme , biology , ecology
A Gram‐ negative rod‐shaped bacterium 28/1 isolated by enrichment cultures is able to hydrolyze the amide bond of some phenylurea herbicides and acid anilide herbicides by an inducible amidase. 7.5% of 0.3 μmol. ml −1 linuron (3‐(3,4‐dichlorophenyl)‐1‐methoxy‐1‐methylurea) are hydrolyzed after 16 hours. 1,1‐Dimethylphenylureas are not degraded. Acid anilides are hydrolyzed at a higher rate, 80% of 0.5 μmol. ml −1 N‐(4‐chlorophenyl)‐propionamide and N‐(4‐nitrophenyl)‐propionamide are transformed after 6 hours. The 1‐methoxy‐1‐methyl phenylureas are effective inducers. Linuron‐induced cells have a specific activity of 3—4 nmol per mg dry weight per min on the substrate N‐(3,4‐dichlorophenyl)‐propionamide (Propanil). The rate of hydrolysis is influenced by substituents of the aniline ring and by the structure of the side chain of the acid anilides.