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Studies on microbial transformations XVIII. Microbiological transformation of l‐α‐santonin
Author(s) -
Iida M.,
Hatori Y.,
Yamakawa K.,
Iizuka H.
Publication year - 1981
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19810210804
Subject(s) - streptomyces aureofaciens , acetone , sesquiterpene , chemistry , sesquiterpene lactone , silica gel , stereochemistry , chromatography , streptomyces , benzene , chemical transformation , chemical structure , transformation (genetics) , column chromatography , organic chemistry , bacteria , biology , biochemistry , genetics , gene
The microbial transformation of a sesquiterpene lactone, l‐α‐santonin was carried out by Streptomyces roseochromogenes NRRL‐B‐1233. The product obtained from l‐α‐sanatonin was purified using silica gel column chromatography with 1% acetone‐benzene as the eluant. The molecular weight of the product was 248, estimated by mass spectroanalysis. The product was assignad the chemical structure of 1,2‐dihydro‐l‐α‐santonin on the bases of 1 H and 13 C‐NMR and mass spectro‐analyses. Similar products were also obtained from other actinomycetes. The conversion of l‐α‐santonin to 1,2‐dihydro‐l‐α‐santonin was 28% for Streptomyces roseochromogenes NRRL‐B‐1233, 14% for S. roseochromogenes ATCC 13400, 30.9% for S. aureofaciens KCC‐S‐0008, and 43.0% for S. aureofaciens KCC‐S‐0624, respectively.