Premium
A novel extracellular proteinase from Bacillus pumilus
Author(s) -
Okotore R. O.,
Akinrimisi E. O.,
Ojo M. O.
Publication year - 1981
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19810210706
Subject(s) - bacillus pumilus , chromatography , chemistry , sodium dodecyl sulfate , glycine , extracellular , polyacrylamide gel electrophoresis , gel electrophoresis , molecular mass , amino acid , biochemistry , ion chromatography , proline , electrophoresis , sodium , enzyme , biology , bacteria , organic chemistry , genetics
Bacillus pumilus NCTC AII6/73 produces a novel extracellular alkaline proteinase whose optimum is pH 9.5. The purified product from ion‐exchange chromatography (DEAE‐cellulose) was resolved into two bands by polyacrylamide‐sodium dodecyl sulphate (SDS) gel electrophoresis. These had approximate molecular weights of 15.6 × 10 3 and 11.1 × 10 3 daltons, respectively, and a high content of glycine and proline, comprising roughly 50% of the total amino acid residues.