Premium
Struktur der Zellwandpolysaccharide in der Futtereiweiß‐Hefe Candida spec. H. IV. Struktur der alkalilabilen Oligosaccharide im Mannan‐Protein‐Phosphat‐Komplex
Author(s) -
Grimmecke H.D,
Reuter G.
Publication year - 1981
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19810210306
Subject(s) - chemistry , oligosaccharide , mannose , mannan , threonine , glucosamine , polysaccharide , methylation , serine , stereochemistry , biochemistry , chromatography , enzyme , dna
Mild alkaline degradation of the proteophosphomannan (PPM) under conditions that effect β‐elimination of the residues, O‐glycosidically bound on serine and threonine, release mainly mannose, glucose, mannobioses and mannotrioses with the same structures as those obtained by acetolysis of the polysaccharide component of the PPM. Methylation analysis and paper chromatography demonstrated the structural heterogeneity of the tetra‐, penta‐ and hexasaccharide fractions containing 1,2‐ and 1,6‐linked and 1,2,6‐branched manno‐oligosaccharides. Methylation analysis and acetolysis, paper chromatography and analysis of the carbohydrate composition of the oligosaccharide fractions DP 7–12 and DP 15–19 demonstrated inner core region like structures containing 1,2‐ and 1,6‐linked mannose, 1,2,6‐ and 1,3,6‐branchpoints and N‐acetyl‐d‐glucosamine.