Premium
Struktur der Zellwandpolysaccharide in der Futtereiweiß‐Hefe Candida spec. H. II. Charakterisierung der Bindung des Phosphats am Mannan‐Protein‐Phosphat‐Komplex und Identifizierung der als Phosphodiester gebundenen Mono‐ und Oligosaccharide
Author(s) -
Grimmecke H. D.,
Reuter G.
Publication year - 1981
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19810210206
Subject(s) - chemistry , oligosaccharide , fucose , mannan , phosphodiester bond , monosaccharide , rhamnose , mannose , hydrolysis , biochemistry , arabinose , phosphate , stereochemistry , galactose , polysaccharide , xylose , fermentation , rna , gene
The proteophosphomannan (PPM) obtained by extraction with citrate buffer, purification by the cetavlon method and gelfiltration contains a high proportion of diesterfied phosphate groups between the C‐6 of a side chain mannose and another glycosidically bound carbohydrate. Mild acid hydrolysis cleaved the phosphate diester linkages to yield mono‐ and oligosaccharide fractions. Chemical analysis and 1 H‐NMR studies demonstrated the heterogeneity of the oligosaccharide fractions containing α(1,2)‐, α(1,3)‐ and α(1,6)‐linked manno‐oligosaccharides. All repeated PPM preparations contained mono‐, tri‐ and heptasaccharides as the phosphate bound main compounds. Galactose, arabinose, fucose, and rhamnose of the monosaccharide fraction and tetra‐, penta‐ and hexasaccharides were not found in all preparations.