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Steroidtransformation with immobilized microorganisms VI. The reverse reaction of steroid‐1‐dehydrogenases from different micoorganisms in immobilized state
Author(s) -
Atrat P.,
Groh H.
Publication year - 1981
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19810210102
Subject(s) - chemistry , cellulose , steroid , adsorption , substrate (aquarium) , oxidoreductase , microorganism , chromatography , biochemistry , enzyme , organic chemistry , bacteria , biology , ecology , genetics , hormone
Whole cells of Nocardia erythropolis, N. opaca and Mycobacterium phlei containing 4‐en‐3‐oxosteroid: (acceptor)‐l‐en‐oxidoreductase activities were immobilized by adsorption on DEAE cellulose and silica and by entrapment in polyacrylamide gel. The obtained biocatalysts were used in an anaerobic continuous column process to transform l,4‐dien‐3‐oxo‐steroids into 4‐en‐3‐oxo‐steroids. The half life of steroid‐1(2)‐reductase activity of the N. erythropolis was found to be up to 15 days. The DEAE‐cellulose‐adsorbed cells showed a slighthly higher relative activity than free cells. The influence of substrate concentration and the action of dimethylformamide were also investigated.