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Experimentelle Induktion von „rotund bodies”︁ bei Thermus aquaticus
Author(s) -
Kraepelin G.,
Grävenstein H.U.
Publication year - 1980
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19800200105
Subject(s) - biophysics , chemistry , lysozyme , swelling , biology , anatomy , biochemistry , materials science , composite material
With several isolates of T. aquaticus rotund bodies (rbs) have been artificially induced. The most active agents were lysozyme, pronase (not trypsin), cellulase (not α‐amylase or β‐glucosidase), penicillin and some mineral salts. Two modifications of rbs were observed: the vesicular type arising from single cells or filaments and the aggregate‐rbs resulting from secondary associations of several cells or filaments. In both cases the primary event is a partial detachment of the outer layer of cell envelopes followed by its swelling to form a hyaline bleb. Within these osmotically stable blebs cells and/or filaments involved in the rbs production become irreversibly trapped. Appearance of filaments and rbs in stationary phase cultures are discussed as a consequence of unbalanced growth culminating in abnormal autolytic reactions.