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Unterschiedliche Empfindlichkeit der Phagen T3, T7, T4 und Lambda gegen Bleomycin und Phleomycin
Author(s) -
Scholz D.,
Meissner Ch.,
Rosenthal H. A.
Publication year - 1979
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19790191010
Subject(s) - bleomycin , lambda phage , dna , in vivo , in vitro , biology , escherichia coli , microbiology and biotechnology , dna synthesis , biochemistry , genetics , bacteriophage , chemotherapy , gene
In contrast to phage lambda the phages T3, T7 and T4 are not inhibited by as much as 150 μg bleomycin/ml, while the chemically related antibiotic phleomycin increasingly inhibits the propagation of the phages in the order T4‐T3‐lambda. 20 μg phleomycin/ml inhibit T3 by 95%. The resistance against bleomycin is surprising, because 10 μg BM/ml block completely the colony‐forming capacity of the host bacterium. The drug resistance of the phage growth correlates with the weak decrease of phage DNA synthesis, while the host cell DNA synthesis ceases rapidly. In accordance with these data is the in vivo inhibition of Escherichia coli cells and the in vitro degradation of their DNA. However, a contradiction exists between the in vivo resistance of T3 and T4 and the in vitro susceptibility of their DNA against nucleolytical fragmentation by bleomycin. The mechanism of the insensitivity of T3, T7 and T4 against bleomycin is unknown.