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Steroidumwandelnde Enzyme aus Mikroorganismen XI. Reversibilität der Dehydrierungsreaktion der Steroid‐1‐Dehydrogenase aus Nocardia opaca
Author(s) -
Groh H.,
Komel R.,
Deppmeyer V.,
Atrat P.,
Hörhold C.
Publication year - 1979
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19790191007
Subject(s) - chemistry , nocardia , derivative (finance) , enzyme , dehydrogenation , oxidoreductase , dehydrogenase , isoelectric focusing , biochemistry , stereochemistry , biology , bacteria , catalysis , genetics , financial economics , economics
Highly purified preparations of the 4‐en‐3‐oxosteroid: (acceptor)‐1‐en‐oxidoreductase from Nocardia opaca have been investigated in both types of reactions: 1.2‐dehydrogenation of the 4‐en‐3‐oxo‐derivative and 1.2‐hydrogenation of the 1.4‐dien‐3‐oxo‐derivative. It was not possible to separate the hydrogenating activity from the dehydrogenating activity by affinity chromatography, disc electrophoresis, SDS‐electrophoresis, and isoelectric focusing techniques. The pure enzyme preparation is discussed as only one FAD depending protein acting in response to the system as a dehydrogenase as well as a reductase.