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Biosynthese der Zellwand‐Polysaccharide Mannan und Glucan als Spiegelbild unterschiedlicher Abbauwege der Glucose durch Candida spec. H
Author(s) -
Róber B.,
Reuter G.
Publication year - 1979
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19790190306
Subject(s) - pentose phosphate pathway , hexose , pentose , mannan , chemistry , biochemistry , catabolism , yeast , mannose , glucan , carbohydrate metabolism , metabolism , anabolism , polysaccharide , fermentation , glycolysis , enzyme
The catabolic and anabolic D ‐glucose transformation of the yeast Candida spec. H has been studied. By using 1‐ 14 C‐ D ‐glucose and 6‐ 14 C‐ D ‐glucose, measuring the 14 CO 2 liberation and the label of glucose and mannose isolated from glucan and mannan, the following results have been obtained. 1. Beginning with 100 μ moles glucose · ml −1 in the batch growth medium, at first on an average 64% of the glucose having been catabolized to CO 2 are directly decarboxylated to pentose phosphate by pentose phosphate pathway (PPW). Later on at an exogen concentration of 70 μ moles · ml −1 73% of glucose on an average having been catabolised to CO 2 undergoes transformation via glycolyse and tricarbonacid cycle (G‐TCC). 2. Only after getting this glucose concentration the maximal hexose incorporation rate into glucan and mannan can be obtained. 3. 20–40% of the hexose channeled into the polysaccharid‐biosynthesis have been prepared by resynthesis from pentose phosphate via. PPW. 4. The results are discussed in connection with the observed crabtree‐effect.