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Phenol oxidase activity and pigment synthesis in conidiospores of Penicillium cyclopium
Author(s) -
Bartsch E.,
Lerbs W.,
Luckner M.
Publication year - 1979
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19790190202
Subject(s) - conidium , spore , sephadex , chemistry , pigment , oxidase test , laccase , sodium azide , polyphenol oxidase , penicillium , biochemistry , chromatography , microbiology and biotechnology , botany , biology , enzyme , food science , organic chemistry , peroxidase
Phenol oxidase (PO) Abbreviations: CH cycloheximide, DIECA diethyldithiocarbamate sodium salt, h p. i. hours after inoculation, NL nutrient solution, PAA polyacrylamide, PDA p ‐phenylenediamine dihydrochloride, PO phenol oxidase of Penicillium cycillium belongs to the laccases (E. C. 1. 14.18.1). By PAA‐gel electrophoresis three forms of the enzym were detected. Most of the PO in emerged sporulating cultures accumulates in the cell wall of the conidiospores. However, a high percentage is not bound to the wall constituents and can be easily removed with aqueous solutions. This soluble fraction was 570‐fold enriched by (NH 4 ) 2 SO 4 ‐precipitation, followed by chromatography on sephadex G 200 and on hydroxylapatite‐cellulose. The necessity of PO for conidiospore pigmentation was shown by means of suspended spores whose rates of pigment accumulation resemble those of conidia maturing in situ. Pigmentation is stopped if PO is inhibited by diethyldithiocarbamate. This corresponds of the fact that in strains with heavily pigmented conidiospores PO acitivity is much higher than in strains with weakly pigmented or unpigmented spores. Also the time course of PO accumulation in the latter strains is different from that of the former. Furthermore, in mutant res‐eth 1 which has unpigmented conidiospores, PO isoenzyme no. 3 could not be detected.