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Hydrostatic pressure effects upon cellular leakage and active transport by Vibrio marinus
Author(s) -
Masuda K. V.,
Albright L. J.
Publication year - 1978
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19780181005
Subject(s) - hydrostatic pressure , valine , leucine , biochemistry , efflux , biophysics , substrate (aquarium) , biology , amino acid , chemistry , ecology , thermodynamics , physics
Hydrostatic pressures greater than ca . 400 atm cause release of cellular components of Vibrio marinus MP‐1 in the order protein > RNA > malate dehydrogenase > DNA > amino acids. Increased pressures also slow the rate of cycloleucine (non‐metabolizable analogue of l‐leucine and l‐valine) uptake but increase its efflux rate. LINEWEAVER ‐ BURK plots (1/ V versus 1/[ S ]) of cycloleucine uptake indicate that the K m increases with compression which suggests a decrease in affinity of the transport system for substrate at increased pressures.
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