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Unterschiede im Prolinstoffwechsel eines Peptidalkaloide‐produzierenden und eines nichtproduzierenden Stammes von Claviceps purpurea
Author(s) -
Schlee D.,
Böhmer SylviaAnnette
Publication year - 1978
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19780180905
Subject(s) - claviceps purpurea , proline , amino acid , biosynthesis , strain (injury) , incubation , chemistry , biochemistry , biology , enzyme , anatomy
Two strains (pepty 695 and pur 221) of Claviceps purpurea have been used to study the dependence of proline turnover on incubation time (1, 3, 6, and 12 hours) under different culture conditions (synthetic saccharose‐citrate medium NL 720 and complex wort medium M 107) by means of tracer technique. In the saprophytically ergotoxine producing strain pepty 695 the proline is utilized in the protein and alkaloid biosyntheses. On the other hand, under non‐producing conditions (M 10) the radioactivity of proline‐ 14 C is incorporated into many fractions and finally into CO 2 . In submerged culture (NL 720) the Claviceps strain pur 221 which does not produce ergolines uses proline only to a small extent in the protein biosynthesis, while most of this amino acid is not metabolized. The extent of uptake of proline is not correlated with the alkaloid synthesis. For comparison the turnover of l‐glutamate‐ 14 C and d, l‐ornithine‐1‐ 14 C by strain pepty 695 cultivated in the NL 720 medium has been studied.