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An alteration in outer membrane permeability associated with a division lesion in a strain of Salmonella typhimurium
Author(s) -
Ahmed N.,
Rowbury R. J.
Publication year - 1978
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19780180702
Subject(s) - lysozyme , strain (injury) , lysis , bacterial outer membrane , cell envelope , biology , salmonella , microbiology and biotechnology , mutant , lipopolysaccharide , phospholipid , peptidoglycan , membrane , chemistry , biochemistry , cell wall , bacteria , escherichia coli , anatomy , immunology , genetics , gene
Salmonella typhimurium strain 4a is a temperature sensitive mutant with defects in both septation and separation. The separation lesion was reversed by phenethylalcohol but this agent failed to allow septation or growth at restrictive temperature. Organisms of strain 4a grown at 42 °C were, unlike the parental strain, resistant to lysis by lysozyme plus EDTA and lipopolysaccharide was poorly extracted by EDTA from cultures of strain 4a grown at 42 °C. Such cultures may, therefore, be resistant to lysis with lysozyme plus EDTA not because the murein is altered but because the EDTA fails to permeabilize the outer membrane to lysozyme. In confirmation of this, murein isolated from strain 4a after growth at 42 °C showed the same sensitivity to lysozyme as murein from the parental strain. In spite of the altered envelope properties of strain 4a after growth at 42 °C, no major changes in protein or phospholipid composition have so far been demonstrated.