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Nachweis von α‐Mannosidase und β‐Glucosidase in einem für die Hefezellwand lytischen System von Streptomyces spec. 5s
Author(s) -
Rademacher K. H.,
Reuter G.
Publication year - 1978
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19780180204
Subject(s) - chromatography , chemistry , lytic cycle , ammonium sulfate , enzyme , streptomyces , mannosidase , ammonium , paper chromatography , biochemistry , biology , bacteria , organic chemistry , virus , genetics , virology
Streptomyces spec. 5s accumulates in the culture fluid lytic activities against living cells of Candida guilliermondii H. From the culture fluid a freeze‐dried dialyzed ammonium sulfate precipitate was prepared. Except β‐glucosidase‐activity it showed activity of α‐mannosidase which was characterized. Separation of both enzymes by chromatography on polyacrylamide gel was possible.