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Isolierung und Charakterisierung von DNA‐Abbaumutanten bei Proteus mirabilis : Funktion der Endonuclease I im postmortalen DNA‐Abbau
Author(s) -
Steinborn G.,
Hofemeister J.,
Böhme H.
Publication year - 1977
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19770170606
Subject(s) - proteus mirabilis , endonuclease , mutant , dna , microbiology and biotechnology , chemistry , in vivo , restriction enzyme , biology , biochemistry , escherichia coli , gene , genetics
DNase deficient mutants of Proteus mirabilis selected for reduced toluene induced DNA degradation were isolated. Their defect in DNA degradation was shown not only after treatment by toluene but also in crude extracts after cell disintegration by ultrasonic and in untreated starved cultures. The degradation mutants behave just as the wild type with respect to their in vivo functions proofed. The results indicate that the affected DNase does not have an essential function in vivo but acts in postmortem DNA degradation. Probably, the DNase in question concerns the endonuclease I of P. mirabilis described by GOEBEL and HELINSKI (1971 a, b).