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Untersuchungen zum transient‐Verhalten ammoniumlimitierter Chemostatenkulturen von Escherichia coli ML 30
Author(s) -
Müller P. J.,
Bergter F.
Publication year - 1977
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19770170206
Subject(s) - ammonium , chemistry , pyruvic acid , escherichia coli , pyruvate decarboxylation , chemostat , dilution , metabolism , biochemistry , biology , bacteria , physics , thermodynamics , citric acid cycle , organic chemistry , gene , genetics
In connection with the bistability of pyruvate formation in ammonium limited continuous cultures of E. coli ML 30 (BERGTER u. ROTH 1977) the transient behaviour of cell density and pyruvate concentration were studied. Immediately after a shift up in the dilution rate from D = 0.15 h −1 to D = 0.6 h −1 the bacteria excreted pyruvate into the medium, followed by a resumption of pyruvate. The specific pyruvate formation rate as well as the specific growth rate reached the new steady state with damped oscillations. Possibly the excretion of pyruvate after the shift is caused by the higher non limiting concentrations of ammonium during the first of the transition. This hypothesis is supported by the transient behaviour of an ammonium limited continuous culture after a pulse of ammonium to the culture. The relations between ammonium metabolism and pyruvate formation are discussed.