Premium
Proteinkristalle und Tubuli‐Bündel in Hefezellen VI. Licht‐ und elektronenmikroskopische sowie biochemische Untersuchungen zur Alkoholdehydrogenase‐Aktivität isolierter Kristalle
Author(s) -
Künkel W.
Publication year - 1977
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19770170204
Subject(s) - chemistry
Isolated crystals from Saccharomyces carlsbergensis , stabilized by Cd 2+ or Zn 2+ , retain their enzymatic activity as shown by topochemical reactions. During these reactions the crystals disintegrate characteristically. Stages of this disintegration and deposition of reaction products have been demonstrated by light and electron microscopy. Ground plasmatic and mitochondrial alcoholdehydrogenase has been solubilized by means of 0.01 M EDTA from Zn 2+ stabilized crystals separated by gel electrophoresis and proved to be active.