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Degradation of sulphur nucleotides in cell‐free extracts of Thiobacillus ferrooxidans
Author(s) -
Tuovinen O. H.,
Kelley B. C.,
Nicholas D. J. D.
Publication year - 1976
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19760160708
Subject(s) - adenosine , nucleotide , phosphate , chemistry , hydrolysis , biochemistry , degradation (telecommunications) , sulfur , enzyme , incubation , adenosine monophosphate , adenine nucleotide , organic chemistry , telecommunications , computer science , gene
The cell‐free extracts of Thiobacillus ferrooxidans contain hydrolytic enzymes which cleave sulphate from either adenosine 5′‐sulphatophosphate or adenosine 3′‐phosphate 5′‐sulphatophosphate. These sulphatase activities may be retarded by including either phosphates or adenine nucleotides in the incubation mixtures. Adenosine 3′‐phosphate 5′‐sulphatophosphate and its immediate breakdown product adenosine 3′‐phosphate 5′‐phosphate were degraded at slower rates than adenosine 5′‐sulphatophosphate.